Item no. |
CS-CMS100 |
Manufacturer |
Cell Sciences
|
Amount |
100 ug |
Category |
|
Type |
Antibody Monoclonal |
Applications |
WB |
Clone |
9E7 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG2 |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Similar products |
ELAM1, CD62E, LECAM2, ELAM, ESEL, endothelial adhesion molecule 1, leukocyte endothelial cell adhesion molecule 2, OTTHUMP00000032745 |
Available |
|
Application Notes |
For Western Blot, use a working dilution of 1:500-2000.For Immunohistochemistry (Paraffin sections), use a working dilution of 1:50-200.The optimal concentration should be determined by the user for each specific application. |
Cross-Reactivity |
No cross-reactivity to ICAM and VCAM |
Description |
Mouse Anti-Human E-Selectin/CD62E monoclonal antibody clone 9E7 E-Selectin is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat domains that contain 6 conserved cysteine residues. E-Selectin is a part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. |
Formulation |
Lyophilized from a 0.2 um sterile filtered solution in PBS |
Immunogen |
Recombinant Human E-Selectin/CD62E |
Purification |
Protein G affinity chromatography |
Reconstitution |
Centrifuge vial prior to opening. Add 500 ul sterile PBS to the vial to fully solubilize the antibody to a concentration of 200 ug/ml. |
Storage and Stability |
Lyophilized antibody is stable for 2 years from date of receipt when stored at -80C. Reconstituted antibody can be aliquoted and stored frozen at -20C to -80C for at least six months without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
Specificity |
Recognizes Human E-Selectin/CD62E |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.