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OxiSelect™ Cellular Antioxidant Assay Kit (Green Fluorescence)

OxiSelect™ Cellular Antioxidant Assay Kit (Green Fluorescence)

Item no.	STA-349
Manufacturer	Cell Biolabs
Amount	192 assays
Category	Cell Biology Cell Status Oxidative Stress Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Manufactuers Category	Kit
Manufacturers Product Classification	Oxidative Stress/ Damage->Antioxidant Assays->Cellular Antioxidant Assay
Shipping Temp	Room Temp
Storage Temp	Upon receipt, store the DCFH-DA Probe (1000X) and Quercetin at -20ºC. Avoid multiple freeze/thaw cycles. Store the store the remaining components at 4ºC.
Detection Method	Fluorometric
Product Description	The OxiSelect™ Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
Additional Information	Determine the efficacy of an exogenous antioxidant compound within a cellular environment Each kit provides sufficient reagents to perform up to 192 assays, including standard curve and unknown samples
LMO Content	None

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.