Comparison

IHC Enzyme Antigen Retrieval Reagent European Partner

Item no. BOS-AR0022
Manufacturer Boster
Amount 50 mL (for 500-750 assays)
Category
Type Solution
Format IHC Related Reagent
Applications IHC
Specific against other
ECLASS 10.1 32120508
ECLASS 11.0 32120508
UNSPSC 12000000
Alias Compound digest solution
Similar products Compound digest solution
Available
Storage Conditions
Store at 4C. Avoid multiple freeze-thaw cycles.
Background
The IHC enzyme antigen retrieval compound digest solution contains multiple proteinases that catalyze proteolysis in tissue samples, breaking the proteins into smaller peptide fractions and amino acids. Enzymatic digestion is commonly used in IHC in the PIER method for antigen retrieval to overcome the lost immunoreactivity of epitopes caused by the covalent cross-links that are formed in tissues during formalin fixation. Proteolysis acts to improve the accessibility of antibodies to target antigens by two main mechanisms: by increasing the penetration of immunoreagents into tissue structures as a result of both tissue digestion and relaxation of the rigid protein structure, and by restoring the methylene-bridged antigenic sites. Since proteases exhibit different cleavage site specificity, the combination of multiple proteinases provides a broader spectrum of proteins digestion in a tissue sample. Thus various types of antigenic targets (e.g. protein, glycoprotein, or non-protein, inter- or intracellular antigens) can benefit from this cocktail of digestion enzymes.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 mL (for 500-750 assays)
Available: Out of stock
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