Item no. |
BOS-EKC2143 |
Manufacturer |
Boster
|
Amount |
1 kit, containing two 96-well plates and all necessary reagents |
Category |
|
Type |
Elisa-Kit |
Applications |
ELISA |
Specific against |
Human |
Sensitivity |
>5000 cells |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
ALK tyrosine kinase receptor precursor;Anaplastic lymphoma kinase;CD246;kinase ALK |
Available |
|
Manufacturers Product Category |
Cell-Based ELISA Kits |
Short Description |
The ALK (Phospho-Tyr1604) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ALK (Phospho-Tyr1604) protein expression profile in cells. The kit can be used for measuring the relative amounts of ALK (Phospho-Tyr1604) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ALK (Phospho-Tyr1604). |
Description |
The ALK (Phospho-Tyr1604) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ALK (Phospho-Tyr1604) protein expression profile in cells. The kit can be used for measuring the relative amounts of ALK (Phospho-Tyr1604) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ALK (Phospho-Tyr1604). |
Gene Name |
ALK |
Storage |
Store at 4°C for up to 6 months. |
Sample Type |
Cell lines |
Gene Full Name |
ALK tyrosine kinase receptor |
Molecular Weight |
176442 MW |
Protein Function |
Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y- x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. |
Subcellular Localization |
Cell membrane; Single-pass type I membrane protein. Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway. |
Tissue Specificity |
Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.