Item no. |
BOS-EKC1785 |
Manufacturer |
Boster
|
Amount |
1 kit, containing one 96-well plate and all necessary reagents |
Category |
|
Type |
Elisa-Kit |
Applications |
ELISA |
Specific against |
Human |
Sensitivity |
>5000 cells |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
A-kinase anchor protein 13;AKAP-13;AKAP-Lbc ;Breast cancer nuclear receptor-binding auxiliary protein ;Guanine nucleotide exchange factor Lbc;Human thyroid-anchoring protein 31 ;Lymphoid blast crisis oncogene ;LBC oncogene ;Non-oncogenic Rho GTPase-specific GTP exchange factor;Protein kinase A-anchoring protein 13;PRKA13;p47;AKAP13;BRX , HT31 , LBC; |
Available |
|
Manufacturers Product Category |
Cell-Based ELISA Kits |
Short Description |
The AKAP13 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AKAP13 protein expression profile in cells. The kit can be used for measuring the relative amounts of AKAP13 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AKAP13. |
Description |
The AKAP13 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AKAP13 protein expression profile in cells. The kit can be used for measuring the relative amounts of AKAP13 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AKAP13. |
Gene Name |
AKAP13BRX , HT31 , LBC |
Storage |
Store at 4°C for up to 6 months. |
Gene Full Name |
A-kinase anchor protein 13 |
Molecular Weight |
307550 MW |
Protein Function |
Scaffold protein that plays an important role in assembling signaling complexes downstream of several types of G protein-coupled receptors. Activates RHOA in response to signaling via G protein-coupled receptors via its function as Rho guanine nucleotide exchange factor (PubMed:11546812, PubMed:15229649, PubMed:23090968, PubMed:25186459, PubMed:24993829). May also activate other Rho family members (PubMed:11546812). Part of a kinase signaling complex that links ADRA1A and ADRA1B adrenergic receptor signaling to the activation of downstream p38 MAP kinases, such as MAPK11 and MAPK14 (PubMed:17537920, PubMed:23716597, PubMed:21224381). Part of a signaling complex that links ADRA1B signaling to the activation of RHOA and IKBKB/IKKB, leading to increased NF-kappa-B transcriptional activity (PubMed:23090968). Part of a RHOA-dependent signaling cascade that mediates responses to lysophosphatidic acid (LPA), a signaling molecule that activates G-protein coupled receptors and potentiates transcriptional activation of the glucocorticoid receptor NR3C1 (PubMed:16469733). Part of a signaling cascade that stimulates MEF2C-dependent gene expression in response to lysophosphatidic acid (LPA) (By similarity). Part of a signaling pathway that activates MAPK11 and/or MAPK14 and leads to increased transcription activation of the estrogen receptors ESR1 and ESR2 (PubMed:9627117, PubMed:11579095). Part of a signaling cascade that links cAMP and EGFR signaling to BRAF signaling and to PKA- mediated phosphorylation of KSR1, leading to the activation of downstream MAP kinases, such as MAPK1 or MAPK3 (PubMed:21102438). Functions as scaffold protein that anchors cAMP-dependent protein kinase (PKA) and PRKD1. This promotes activation of PRKD1, leading to increased phosphorylation of HDAC5 and ultimately cardiomyocyte hypertrophy (By similarity). Has no guanine nucleotide exchange activity on CDC42, Ras or Rac (PubMed:11546812). Required for normal embryonic heart development, and in particular for normal sarcomere formation in the developing cardiomyocytes (By similarity). Plays a role in cardiomyocyte growth and cardiac hypertrophy in response to activation of the beta-adrenergic receptor by phenylephrine or isoproterenol (PubMed:17537920, PubMed:23090968). Required for normal adaptive cardiac hypertrophy in response to pressure overload (PubMed:23716597). Plays a role in osteogenesis (By similarity). |
Subcellular Localization |
Cytoplasm, cytosol. Cytoplasm. Cytoplasm, cell cortex. Nucleus. Membrane; Peripheral membrane protein. Colocalizes with the actin cytoskeleton at the cell cortex. |
Tissue Specificity |
Detected in mammary gland (PubMed:9627117). Detected in heart (at protein level) (PubMed:11546812). Expressed as a 5.3 kb transcript in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Predominantly expressed as a 10 kb transcript in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver. Transcripts of between 6-9 kb are also expressed in myeloid and lymphoid lineages, a variety of epithelial tissues, and in skeletal muscle. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.