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Human BMP-2 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Human BMP-2 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Manufacturer	Boster
Category	Oncology Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer
Type	Elisa-Kit
Specific against	Human
Applications	ELISA
Amount	5 plates/kit
Item no.	BOS-EZ0311
Targets	BMP2
eClass 6.1	32160605
eClass 9.0	32160605
Available
Alias	Bone morphogenetic protein 2 ;BMP2 ;
Assay procedure step1	2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml
Background	Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Components needed	Components Included In The EZ Set Accessory Kit Antibody diluent buffer ABC diluent buffer Sample diluent buffer TMB TMB stop solution 5x 96 well plates, plate sealers *The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you. Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Cross reactivity	There is no detectable cross-reactivity with other relevant proteins.
Description	A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BMP-2 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
Gene full name	bone morphogenetic protein 2
Gene name	BMP2
Immunogen	Expression system for standard: CHO; Immunogen sequence: Q283-R396
Intra inter assay cv	\|Intra-Assay Precision\|Inter-Assay Precision Sample\|1\|2\|3\|1\|2\|3 n\|16\|16\|16\|24\|24\|24 Mean(pg/ml)\|285\|742\|1197\|306\|834\|1358 Standard deviation\|9.12\|25.97\|37.12\|18.97\|58.38\|97.78 CV(%)\|3.2\|3.5\|3.1\|6.2\|7\|7.2
Kit components	Catalog number\|Description\|Quantity EZ0311-CA\|Capture Antibody For BMP-2\|500ul EZ0311-DA\|Biotinylated Detection Antibody For BMP-2\|500ul ST0000-10\|lyophilized recombinant human Lipocalin-2/BMP-2 standard\|10ng/tubex2 AR1103\|Avidin-Biotin-Peroxidase Complex(ABC)\|500ul(dilution 1:100)
MW	44702 MW
Normal range citations	25257151
Principle	monoclonal antibody from mouse\|polyclonal antibody from goat
Protein name	Bone morphogenetic protein 2
Research category	\|cardiovascular\|angiogenesis\|growth factors\|tgf\| signal transduction\|growth factors/hormones\|cytoskeleton / ecm\|extracellular matrix\|structures\|bone\| stem cells\|signaling pathways\|tgf beta\|secreted\|neural stem cells\|extracellular\| cancer
Sample data	Concentration(pg/ml)\|0\|31.2\|62.5\|125\|250\|500\|1000\|2000 O.D.\|0.005\|0.034\|0.059\|0.119\|0.277\|0.680\|1.642\|2.417
Sample type	bone tissue, cell culture supernates and serum.
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Tmb Incubation time	15-20min

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5 plates/kit

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