Human ADA/Adenosine Deaminase ELISA Kit PicoKine®

PicoKine® ELISA Kits

Human ADA/Adenosine Deaminase ELISA Kit PicoKine® (96 Tests). Quantitate Human ADA in cell culture supernatants, serum and pleural fluid. Sensitivity: 10pg/ml.

Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

Dilution ratio of 1:1, concentration in serum is around 400 pg/ml.

There is no detectable cross-reactivity with other relevant proteins.

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

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40764 MW

Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.

Adenosine deaminase