Comparison

Anti-EGF Antibody Picoband™ European Partner

Item no. BOS-A00378-1
Manufacturer Boster
Amount 100 ug/vial
Category
Type Antibody Polyclonal
Format Lyophilized
Applications WB, IHC, ELISA
Specific against Rat
Host Rabbit
Isotype IgG
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Pro-epidermal growth factor;EGF;Epidermal growth factor;Egf;
Available
Manufacturers Product Category
Primary Antibodies, Rabbit Polyclonal Antibodies
Manufacturers Research Category
Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction
Short Description
Boster Bio Anti-EGF Antibody Picoband™ catalog # A00378-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.
Description
Boster Bio Anti-EGF Antibody Picoband™ catalog # A00378-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.
Background
EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Gene Name
EGF
Application Details
Western blot, 0.1-0.5μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA(Cap) , 1-5μg/ml, Rat
Application Notes
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Clonality
Polyclonal
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen
E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.
Purification
Immunogen affinity purified.
Cross-reactivity
No cross-reactivity with other proteins.
Storage
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name
Pro-epidermal growth factor
Molecular Weight
124126 MW
Protein Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity)..
Subcellular Localization
Membrane; Single-pass type I membrane protein.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug/vial
Available: In stock
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