Item no. |
BOS-M06819-22 |
Manufacturer |
Boster
|
Amount |
100ug |
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, ELISA |
Clone |
RM171 |
Specific against |
Human |
Host |
Rabbit |
Isotype |
IgG |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Histone H3.3;H3F3A;H3.3A, H3F3;PP781;H3F3B;H3.3B; |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Monoclonal Antibodies |
Manufacturers Research Category |
Epigenetics and Nuclear Signaling, Histones |
Short Description |
Boster Bio Anti-Dimethyl Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM171 (Catalog # M06819-22). Tested in WB, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates. |
Description |
Boster Bio Anti-Dimethyl Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM171 (Catalog # M06819-22). Tested in WB, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates. |
Gene Name |
H3F3A |
Clonality |
Monoclonal |
Concentration |
1 mg/mL |
Contents |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Application Details |
WB: 0.5 ug/mL – 2 ug/mL
ELISA: 0.1 ug/mL - 0.5 ug/mL
Luminex: 0.1 ug/mL – 0.5 ug/mL. |
Immunogen |
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys23). |
Cross-reactivity |
This antibody reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross-reactivity with monomethylated Lysine 23 (K23me1) or trimethylated Lysine 23 (K23me3), or other methylation in histone H3. |
Purification |
Protein A affinity purified from an animal origin-free culture supernatant |
Storage |
Store at -20°C for one year. Avoid repeated freeze-thaw cycles. |
Gene Full Name |
Histone H3.3 |
Molecular Weight |
15328 MW |
Protein Function |
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
Subcellular Localization |
Nucleus. Chromosome. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.