Item no. |
BOS-M02393-2 |
Manufacturer |
Boster
|
Amount |
200ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
WB, FC, IF, IP, ICC |
Clone |
9G10 |
Specific against |
Human, Mouse, Rat, Monkey, Rabbit, Guinea Pig, Sheep, Hamster, Chicken, Dog, Horse, Pig, Cattle |
Host |
Rat |
Isotype |
IgG2a |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
HSP90B1, GP96, TRA1, ECGP, 94 kDa glucose regulated protein, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, ENPL_HUMAN, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 94, GRP-94, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, Heat shock protein, 90 kDa, beta, 1, HSP90B1, Stress inducible tumor rejection antigen GP96, TRA1, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen gp96, Tumor rejection antigen-1 (gp96) |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Monoclonal Antibodies |
Manufacturers Research Category |
Tags & Cell Markers |
Short Description |
Boster Bio Anti-GRP94 Monoclonal Antibody catalog # M02393-2. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-GRP94 Monoclonal Antibody catalog # M02393-2. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7). |
Gene Name |
GRP94 |
Clonality |
Monoclonal |
Concentration |
1 mg/ml |
Contents |
PBS pH7.2, 50% glycerol, 0.09% sodium azide |
Application Details |
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
Immunogen |
Purified Grp94 isolated from chicken oviducts |
Cross-reactivity |
Detects ~98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli. |
Purification |
Protein G Purified |
Storage |
Store at -20°C for one year. Avoid repeated freeze-thaw cycles. |
Subcellular Localization |
Endoplasmic Reticulum| Endoplasmic Reticulum Lumen. Melanosome |
Protein Name |
GRP94 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.