Comparison

Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody European Partner

Item no. BOS-M01432
Manufacturer Boster
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications FC, IF, IHC
Clone KRT14/532
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Mouse
Isotype IgG3 Kappa
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Keratin, type I cytoskeletal 14;Cytokeratin-14;CK-14;Keratin-14;K14;KRT14;
Available
Manufacturer - Category
Primary Antibodies, Monoclonal Antibodies, 2279
Manufacturer - Isotype
IgG3, kappa
Storage Conditions
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Observed Molecular Weight
51561 MW
Clonality
Monoclonal
Application Details
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Manufacturer - Gene Name
KRT14
Gene Full Name
Keratin, type I cytoskeletal 14
Background
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Immunogen
Recombinant full-length human KRT14 protein
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purification
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.
Concentration
Purified antibody with BSA and azide at 200ug/ml
Protein Function
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Subcellular Localization
Cytoplasmic
Short Description
Boster Bio Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody (Catalog # M01432). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Description
Boster Bio Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody (Catalog # M01432). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close