Item no. |
BOS-M01432 |
Manufacturer |
Boster
|
Amount |
100 ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
FC, IF, IHC |
Clone |
KRT14/532 |
Specific against |
Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus) |
Host |
Mouse |
Isotype |
IgG3 Kappa |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Keratin, type I cytoskeletal 14;Cytokeratin-14;CK-14;Keratin-14;K14;KRT14; |
Available |
|
Manufacturer - Category |
Primary Antibodies, Monoclonal Antibodies, 2279 |
Manufacturer - Isotype |
IgG3, kappa |
Storage Conditions |
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
Observed Molecular Weight |
51561 MW |
Clonality |
Monoclonal |
Application Details |
Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. |
Manufacturer - Gene Name |
KRT14 |
Gene Full Name |
Keratin, type I cytoskeletal 14 |
Background |
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast. |
Immunogen |
Recombinant full-length human KRT14 protein |
Contents |
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Purification |
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G. |
Concentration |
Purified antibody with BSA and azide at 200ug/ml |
Protein Function |
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. |
Subcellular Localization |
Cytoplasmic |
Short Description |
Boster Bio Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody (Catalog # M01432). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Cytokeratin 14 (KRT14) (Squamous Cell Marker) Monoclonal Antibody (Catalog # M01432). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat. |
Tissue Specificity |
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.