Item no. |
BOS-M01340-1 |
Manufacturer |
Boster
|
Amount |
100ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
WB, FC, IF, IHC |
Clone |
LN-2 |
Specific against |
Human, Mouse |
Host |
Mouse |
Isotype |
IgG1 Kappa |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Monoclonal Antibodies |
Manufacturers Research Category |
Adaptive Immunity, B Cells, Immunology |
Short Description |
Boster Bio Anti-CD74 (B-Cell Marker) Monoclonal Antibody (Catalog # M01340-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Baboon, Mouse. |
Description |
Boster Bio Anti-CD74 (B-Cell Marker) Monoclonal Antibody (Catalog # M01340-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Baboon, Mouse. |
Background |
This monoclonal antibody recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). |
Gene Name |
CD74 |
Clonality |
Monoclonal |
Concentration |
Purified antibody with BSA and azide at 200ug/ml |
Contents |
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Application Details |
Flow Cytometry (1-2ug/million cells) (Methanol Fixed)Immunofluorescence (1-4ug/ml) Western Blot (2-4ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. |
Immunogen |
SU-DHL-4 lymphoma cells |
Purification |
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G. |
Storage |
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
Subcellular Localization |
Cell surface and paranuclear globular |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.