Item no. |
BOS-M00936 |
Manufacturer |
Boster
|
Amount |
100 ug |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
FC, IF, IHC |
Clone |
SPM319 |
Specific against |
Human (Homo sapiens) |
Host |
Mouse |
Isotype |
IgG1 Kappa |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Tenascin;TN;Cytotactin;GMEM;GP 150-225;Glioma-associated-extracellular matrix antigen;Hexabrachion;JI;Myotendinous antigen;Neuronectin;Tenascin-C;TN-C;TNC;HXB; |
Available |
|
Manufacturer - Category |
Primary Antibodies, Monoclonal Antibodies, 2279 |
Storage Conditions |
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
Observed Molecular Weight |
240853 MW |
Clonality |
Monoclonal |
Application Details |
Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined. |
Manufacturer - Gene Name |
TNC |
Gene Full Name |
Tenascin |
Background |
In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of This monoclonal antibody is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas. |
Immunogen |
Human breast carcinoma |
Contents |
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Purification |
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G. |
Concentration |
Purified antibody with BSA and azide at 200ug/ml |
Manufacturer - Research Category |
Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Neural Signal Transduction, Neural Stem Cells, Neurology Process, Neuroscience, Secreted Molecules, Signal Transduction, Stem Cells |
Protein Function |
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. |
Subcellular Localization |
Connective tissue matrix |
Short Description |
Boster Bio Anti-Tenascin C (Stromal Marker For Epithelial Malignancy) Monoclonal Antibody (Catalog # M00936). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-Tenascin C (Stromal Marker For Epithelial Malignancy) Monoclonal Antibody (Catalog # M00936). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human. |