Item no. |
BOS-M00458-1 |
Manufacturer |
Boster
|
Amount |
100 ug/vial |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB, IHC |
Clone |
1A3/2A10 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG1 |
Citations |
1. Abramson, J. S., Mills, E. L., Sawyer, M. K., Regelmann, W. R., Nelson, J. D., Quie, P. G. Recurrent infections and delayed separation of the umbilical cord in an infant with abnormal phagocytic cell locomotion and oxidative response during particle phagocytosis. J. Pediat. 99: 887-894, 1981.<br/>2. Akao, Y., Utsumi, K. R., Naito, K., Ueda, R., Takahashi, T., Yamada, K. Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1 beta subunit. Somat. Cell Molec. Genet. 13: 273-278, 1987.<br/>3. "Entrez Gene: ITGB2 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)". |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD18; ITGB2; CD18; MFI7 |
Available |
|
Specificity |
No cross reactivity with other proteins. |
Manufacturers Product Category |
Primary Antibodies, Mouse Monoclonal Antibodies |
Manufacturers Research Category |
Cell Adhesion, Cell Type Markers, Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells |
Short Description |
Boster Bio Anti-CD18 ITGB2 Antibody Picoband™ (monoclonal, 1A3/2A10) catalog # M00458-1. Tested in IHC, WB applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-CD18 ITGB2 Antibody Picoband™ (monoclonal, 1A3/2A10) catalog # M00458-1. Tested in IHC, WB applications. This antibody reacts with Human. |
Background |
ITGB2 (INTEGRIN, BETA-2), also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. ITGB2 is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The ITGB2 gene is mapped to 21q22.3. The expression of CD18 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of ITGB2 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although ITGB2 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways. |
Gene Name |
ITGB2 |
Application Details |
Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml |
Clonality |
Monoclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18. |
Cross-reactivity |
No cross-reactivity with other proteins. |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
Molecular Weight |
85 kDa |
Protein Function |
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). |
Subcellular Localization |
Membrane; Single-pass type I membrane protein. |
Tissue Specificity |
Leukocytes. |
Protein Name |
Integrin beta-2 |
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