Item no. |
BOS-M00123 |
Manufacturer |
Boster
|
Amount |
100 ul |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
WB, IF, IP, IHC, ICC |
Clone |
DBH-2 |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Bromodomain-containing protein 4;Protein HUNK1;BRD4;HUNK1; |
Available |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Monoclonal Antibodies |
Manufacturers Research Category |
Cancer, Chromatin Binding Proteins, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Transcription, Tumor Suppressors |
Short Description |
Boster Bio Anti-Brd4 Rabbit Monoclonal Antibody catalog # M00123. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Brd4 Rabbit Monoclonal Antibody catalog # M00123. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. |
Gene Name |
BRD4 |
Application Details |
WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:50 |
Clonality |
Monoclonal |
Concentration |
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Contents |
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
Immunogen |
A synthesized peptide derived from human Brd4 |
Purification |
Affinity-chromatography |
Storage |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Gene Full Name |
Bromodomain-containing protein 4 |
Molecular Weight |
152219 MW |
Protein Function |
Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P- TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P- TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.. |
Subcellular Localization |
Nucleus. Chromosome. Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment. |
Tissue Specificity |
Ubiquitously expressed.. |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.