Item no. |
MB9001-100 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
100ul |
Category |
|
Type |
Antibody Monoclonal |
Applications |
FC, IF, ELISA |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
BrdU, Bromodeoxyuridine |
Similar products |
BrdU |
Available |
|
Application all |
ELISA: 0.015ca. 0.05 ug/ml Flow cytometry: 1ca.3 ug for 1x106 cells ICC/IF: 1ca.3 ug/ml |
Background |
The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdUrd immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid. |
Product |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Purification and Purity |
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
The antibody is stable in lyophilized form if stored at -20C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8C. For long term storage, aliquot and store at -20C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2'-deoxyuridine (BrdU), 5-chloro-2'-deoxyuridine (CldU) and 5-iodo-2'-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2'-deoxyuridine (FdU) and thymidine (T). |
IHC |
Immunocytochemistry/Immunofluorescence analysis of Hela cells incorporated with BrdU using BrdU mAb, MB9001 |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
BrdU, Bromodeoxyuridine |
Immunogen |
Recombinant full length Human BrdU. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.