P51397

In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved cytoplasmic region called the "death domain". DAP-1 (for death associated protein-1) interacts with the death domain of TNF-R1 and can trigger programmed cell death in a variety of cell lines, as well as suppress NFkappaB/Rel activity. DAP-3 is a positive mediator of programmed cell death, overexpressed intact full-length protein is required in order to induce apoptosis. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

DAP pAb detects endogenous levels of DAP protein.

Immunofluorescence analysis of MCF7 cells, using DAP polyclonal antibody

For research use only, not for use in diagnostic procedure.

Recombinant full length Human DAP.