Item no. |
BS7941-100 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
100ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IHC |
Specific against |
Human, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
DAP 1, Dap, DAP-1, DAP1_HUMAN, Death Associated Protein 1, Death associated protein, Death-associated protein 1, MGC99796, OTTHUMP00000161670, OTTHUMP00000221331, |
Similar products |
DAP |
Available |
|
Swiss-Prot |
P51397 |
Application all |
WB: 1:500ca.1:2000 IHC: 1:50ca.1:200 |
Background |
In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved cytoplasmic region called the "death domain". DAP-1 (for death associated protein-1) interacts with the death domain of TNF-R1 and can trigger programmed cell death in a variety of cell lines, as well as suppress NFkappaB/Rel activity. DAP-3 is a positive mediator of programmed cell death, overexpressed intact full-length protein is required in order to induce apoptosis. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways. |
Product |
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
DAP pAb detects endogenous levels of DAP protein. |
WB |
WesternBlot (WB) analysis of DAP polyclonal antibody |
IF |
Immunofluorescence analysis of MCF7 cells, using DAP polyclonal antibody |
BiowMW |
ca. 11 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
DAP 1, Dap, DAP-1, DAP1_HUMAN, Death Associated Protein 1, Death associated protein, Death-associated protein 1, MGC99796, OTTHUMP00000161670, OTTHUMP00000221331, |
Immunogen |
Recombinant full length Human DAP. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.