Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Rat |
Applications |
WB, IF, IHC |
Amount |
50ul |
Host |
Rabbit |
Item no. |
BS4310-50 |
Targets |
EGF ;;ABL1;;EGFR |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
Epidermal growth factor receptor, EC=2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1, EGFR |
Swiss-Prot |
P00533 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200 |
Background |
Epidermal growth factor mediates its effects on cell growth through its interaction with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGFalpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy-terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptorgulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. |
Product |
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
p-EGFR (Y992) pAb detects endogenous levels of EGFR protein only when phosphorylated at Tyr992 (removal of the signal peptide). |
WB |
Western blot (WB) analysis of p-EGFR (Y992) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate treated with EGF(0.1ng/ml, 30mins) Lane2:HepG2 whole cell lysate treated with EGF(0.1ng/ml, 30mins) Lane3:Rat liver tissue lysate |
IHC |
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p-EGFR (Y992) pAb. The picture on the right is treated with the synthesized peptide. |
IF |
Immunofluorescence analysis of HeLa cells, using p-EGFR (Y992) pAb. The picture on the right is treated with the synthesized peptide. |
BiowMW |
ca. 175 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Epidermal growth factor receptor, EC=2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1, EGFR |
Immunogen |
Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 992. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.