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Histone H4 (aDi-Methyl R3) polyclonal antibody

Histone H4 (aDi-Methyl R3) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Neuroscience Neural Epigenetics Protein Aggregation, Clearance & Misfolding Antibodies Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Applications	WB, IF, IP, IHC
Amount	50ul
Host	Rabbit
Item no.	BS7246-50
Targets	CCDC6;;H4C1
eClass 6.1	32160702
eClass 9.0	32160702
Available
Alias	H4R3me2a, H4R3me2
Swiss-Prot	P62805
Application all	WB: 1:500ca.1:2000 IHC/IF: 1:50ca.1:200 IP: 1:50ca.1:200
Background	Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.
Product	1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	Histone H4 (aDi-Methyl R3) pAb detects endogenous levels of histone H4 only when asymmetric Di-Methyl at Arg3.
WB	Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using Histone H4 (aDi-Methyl R3) antibody.
IHC	Dot-blot analysis of all sorts of methylation peptides using Histone H4 (aDi-Methyl R3) antibody.
IF	Immunofluorescence analysis of 293T cell using Histone H4 (aDi-Methyl R3) antibody. Blue: DAPI for nuclear staining.
BiowMW	ca. 11 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	H4R3me2a, H4R3me2
Immunogen	Synthetic peptide

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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