Item no. |
BS60446-50 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
50ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, Short name=RF-A protein 1, Single-stranded DNA-binding protein, Cleaved into the following chain:Replication protein A 70 kDa DNA-binding subunit, N-terminally pro |
Similar products |
RPA1 |
Available |
|
Swiss-Prot |
P27694 |
Application all |
WB: 1:500ca.1:1000 |
Background |
The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a hetero-trimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
RPA1 pAb detects endogenous levels of RPA1 protein. |
WB |
Western blot (WB) analysis of RPA1 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate |
BiowMW |
ca. 195 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, Short name=RF-A protein 1, Single-stranded DNA-binding protein, Cleaved into the following chain:Replication protein A 70 kDa DNA-binding subunit, N-terminally processed, RPA1, REPA1, RPA70 |
Immunogen |
A synthetic peptide corresponding to residues in Human RPA1. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.