Item no. |
BS60017-100 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
100ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
SPARC, Basement-membrane protein 40, BM-40, Osteonectin, Short name=ON, Secreted protein acidic and rich in cysteine, SPARC, ON |
Similar products |
SPARC |
Available |
|
Swiss-Prot |
P09486 |
Application all |
WB: 1:500ca.1:1000 |
Background |
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules. |
Product |
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
SPARC pAb detects endogenous levels of SPARC protein. |
WB |
Western blot (WB) analysis of SPARC pAb at 1:500 dilution Lane1:A549 whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate |
BiowMW |
ca. 35 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
SPARC, Basement-membrane protein 40, BM-40, Osteonectin, Short name=ON, Secreted protein acidic and rich in cysteine, SPARC, ON |
Immunogen |
A synthetic peptide corresponding to residues in Human SPARC. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.