Item no. |
BS4853-100 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
100ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Ribosomal protein S6 kinase I, S6K, S6K1, 70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase alpha, p70 S6K, p70-S6K, P70S6K, p70 S6 kinase alpha, Name: RPS6KB1, STK14A, p80 S6K1, p85 S6 kinase, p85 S6K, p85S6K |
Similar products |
p70 S6K |
Available |
|
Swiss-Prot |
P23443 |
Application all |
WB:1:500ca.1:1000 IHC:1:50ca.1:200 |
Background |
p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal.Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region. Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression. Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase. |
Product |
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
p-p70 S6K (T421) pAb detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr421. This antibody also detects p85 S6 kinase when phosphorylated at Thr444. |
WB |
Western blot (WB) analysis of p-p70 S6K (T421) pAb at 1:500 dilution Lane1:HEK293T cell lysate treated with PMA(100nM, 15mins) Lane2:NIH-3T3 cell lysate treated with PMA(100nM, 15mins) Lane3:H9C2 cell lysate treated with PMA(100nM, 15mins) |
BiowMW |
ca. 59, 70, 85 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Ribosomal protein S6 kinase I, S6K, S6K1, 70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase alpha, p70 S6K, p70-S6K, P70S6K, p70 S6 kinase alpha, Name: RPS6KB1, STK14A, p80 S6K1, p85 S6 kinase, p85 S6K, p85S6K |
Immunogen |
Synthetic phosphopeptide derived from human p70 S6K around the phosphorylation site of Threonine 421. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.