Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB, IHC |
Amount |
100ul |
Host |
Rabbit |
Item no. |
BS4474-100 |
Targets |
BAD ;;BCL2;;BCL2L1 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
BAD, Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8 |
Swiss-Prot |
Q92934 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
p-Bad (S91) pAb detects endogenous levels of Bad protein only when phosphorylated at Ser91. |
WB |
Western blot (WB) analysis of p-Bad (S91) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:Rat brain tissue lysate |
IHC |
Immunohistochemistry (IHC) analyzes of p-Bad (S91) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 24 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
BAD, Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8 |
Immunogen |
Synthetic phosphopeptide derived from human Bad around the phosphorylation site of Serine 91. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.