Item no. |
BS4346-100 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
100ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA |
Similar products |
PKC alpha |
Available |
|
Swiss-Prot |
P17252 |
Application all |
WB: 1:500ca.1:1000 |
Background |
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
p-PKC a/a2 (T638) pAb detects endogenous levels of PKC a/a2 protein when phosphorylated at Thr638. |
WB |
Western blot (WB) analysis of p-PKC a/a2 pAb in extracts from raw264.7 and hela cells.
|
BiowMW |
ca. 80 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA |
Immunogen |
Synthetic phosphopeptide derived from human PKC alpha around the phosphorylation site of Threonine 638. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.