Cbl (phospho-Y774) polyclonal antibody

P22681

Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL , Casitas B-lineage lymphoma proto-oncogene and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias, c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4, 11) or t(11, 14) abnormalities.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

p-Cbl (Y774) pAb detects endogenous levels of Cbl only when phosphorylated at Tyr774.

Immunohistochemistry (IHC) analysis of p-Cbl (Y774) pAb in paraffin-embedded human breast carcinoma tissue.

For research use only, not for use in diagnostic procedure.

Synthetic phosphopeptide derived from human Cbl around the phosphorylation site of Tyrosine 774.