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NFkB-p65 (phospho-S276) polyclonal antibody

NFkB-p65 (phospho-S276) polyclonal antibody

Item no.	BS4135-100
Manufacturer	Bioworld Technology, Inc
Amount	100ul
Category	Neuroscience Aging & Neurological Disorders Antibodies Immunohistochemistry Immunoprecipitation Western Blot Primary Antibodies
Type	Antibody Polyclonal
Applications	WB, IP, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	p65, p65 NF kappaB, p65 NFkB, RELA, NFKB P65, Transcription Factor p65, Nuclear factor NF-kappa-B p65 subunit, NFkB-p65, NFKB3
Similar products	NFkB-p65
Available
Swiss-Prot	Q04206
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IP: 1:50ca.1:200
Background	p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	p-NFkappaB-p65 (S276) pAb detects endogenous levels of NFkappaB-p65 protein only when phosphorylated at Ser276.
WB	Western blot (WB) analysis of p-NFkB-p65 (S276) pAb at 1:500 dilution Lane1:Hela cell lysate treated with TNFa Lane2:Mouse kidney tissue lysate Lane3:PC12 cell lysate treated with UV
IHC	Immunohistochemistry (IHC) analyzes of p-NFkB-65 (S468) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
BiowMW	ca. 60, 75 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	p65, p65 NF kappaB, p65 NFkB, RELA, NFKB P65, Transcription Factor p65, Nuclear factor NF-kappa-B p65 subunit, NFkB-p65, NFKB3
Immunogen	Synthetic phosphopeptide derived from human NFkB-p65 around the phosphorylation site of Serine 276.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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