Item no. |
BS3762-50 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
50ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Beta-adrenergic receptor kinase 1, Beta-ARK-1, beta-ARK-1, ARK-beta1, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2, ARK beta1, ARKbeta1, beta-ARK1 |
Similar products |
GRK 2 |
Available |
|
Swiss-Prot |
P25098 |
Application all |
WB: 1:500ca.1:1000
IHC: 1:50ca.1:200 |
Background |
Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second messenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1, two forms of beta-adrenergic receptor kinase, GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
GRK 2 (V678) pAb detects endogenous levels of GRK 2 protein. |
IHC |
Immunohistochemistry (IHC) analyzes of GRK 2 (V678) pAb in paraffin-embedded human breast carcinoma tissue at 1:50. |
BiowMW |
ca. 80 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Beta-adrenergic receptor kinase 1, Beta-ARK-1, beta-ARK-1, ARK-beta1, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2, ARK beta1, ARKbeta1, beta-ARK1 |
Immunogen |
Synthetic peptide, corresponding to amino acids 650-698 of Human GRK 2. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.