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MEK1 (T286) polyclonal antibody

MEK1 (T286) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Neuroscience Neural Signaling Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Applications	WB, IHC
Amount	100ul
Host	Rabbit
Item no.	BS3662-100
Targets	MAP2K1;;MAPK1
eClass 6.1	32160702
eClass 9.0	32160702
Available
Alias	Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK-1, PRKMK1
Swiss-Prot	Q02750
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200
Background	MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	MEK1 (T286) pAb detects endogenous levels of MEK1 protein.
WB	Western blot (WB) analysis of MEK1 (T286) pAb at 1:500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
IHC	Immunohistochemistry (IHC) analyzes of MEK1 (T286) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
BiowMW	ca. 43 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK-1, PRKMK1
Immunogen	Synthetic peptide, corresponding to amino acids 260-310 of Human MEK1.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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