Item no. |
BS3635-50 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
50ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Ribosomal protein S6 kinase I, S6K, S6K1, 70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase alpha, p70 S6K, p70-S6K, P70S6K, p70 S6 kinase alpha, Name: RPS6KB1, STK14A, p80 S6K1, p85 S6 kinase, p85 S6K, p85S6K |
Similar products |
p70 S6K |
Available |
|
Swiss-Prot |
P23443 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200 |
Background |
p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal. Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade. The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. |
Product |
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
p70 S6K (S435) pAb detects endogenous levels of p70 S6 Kinase protein. This antibody also recognizes p85 S6 kinase protein. |
WB |
Western blot (WB) analysis of p70 S6K (S435) pAb at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate |
IHC |
Immunohistochemistry (IHC) analyzes of p70 S6K (S435) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 59, 70, 85 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Ribosomal protein S6 kinase I, S6K, S6K1, 70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase alpha, p70 S6K, p70-S6K, P70S6K, p70 S6 kinase alpha, Name: RPS6KB1, STK14A, p80 S6K1, p85 S6 kinase, p85 S6K, p85S6K |
Immunogen |
Synthetic peptide, corresponding to amino acids 400-450 of Human p70 S6K. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.