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AKT1 (P125) polyclonal antibody

AKT1 (P125) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Neuroscience Metabolism Diabetes Aging & Neurological Disorders Neural Signaling Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Applications	WB, IF, IHC
Amount	100ul
Host	Rabbit
Item no.	BS2987-100
Targets	XPC ;;AKT1;;PTK2B
eClass 6.1	32160702
eClass 9.0	32160702
Available
Alias	RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC, PKBalpha
Swiss-Prot	P31749
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200
Background	AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	AKT1 (P125) pAb detects endogenous levels of AKT1 protein, this antibody does not cross-react with AKT2 or AKT3.
WB	Western blot (WB) analysis of AKT1 (P125) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:A549 cell lysate Lane3:NIH-3T3 cell lysate Lane4:PC12 cell lysate
IHC	Immunohistochemistry (IHC) analyzes of Akt1 (P125) pAb in paraffin-embedded human skeletal muscle tissue.
BiowMW	ca. 60 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC, PKBalpha
Immunogen	Synthetic peptide, corresponding to amino acids 100-150 of Human AKT1.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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