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BARD1 (R8) polyclonal antibody

BARD1 (R8) polyclonal antibody

Item no.	BS2408-100
Manufacturer	Bioworld Technology, Inc
Amount	100ul
Category	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Polyclonal
Applications	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	BRCA1-associated RING domain protein 1, BARD-1, BARD 1
Similar products	BARD1
Available
Swiss-Prot	Q99728
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200
Background	The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is an ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	BARD1 (R8) pAb detects endogenous levels of BARD1 protein.
WB	Western blot (WB) analysis of BARD1 (R8) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:Raw264.7 cell lysate Lane3:H9C2 cell lysate
BiowMW	ca. 87 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	BRCA1-associated RING domain protein 1, BARD-1, BARD 1
Immunogen	Synthetic peptide, corresponding to the N-terminual of Human BARD1.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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