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TFIIA-alpha (E311) polyclonal antibody

TFIIA-alpha (E311) polyclonal antibody

Item no.	BS2282-50
Manufacturer	Bioworld Technology, Inc
Amount	50ul
Category	Neuroscience Neuroinflammation Molecular Targets for Neuroscience Antibodies Cell Biology Cell Status Immunohistochemistry Western Blot Senescence Primary Antibodies
Type	Antibody Polyclonal
Applications	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42, Transcription initiation factor IIA alpha chain, TFIIA p35 subunit, Transcription initiation
Similar products	TFIIA-alpha
Available
Swiss-Prot	P52655
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200
Background	Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-alpha, TFIIA-beta and TFIIA-gamma, and it interacts with both TBP and a TAF (TBP-associated factor). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	TFIIA-alpha (E311) pAb detects endogenous levels of TFIIA-alpha protein.
WB	Western blot (WB) analysis of TFIIA-alpha (E311) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
IHC	Immunohistochemistry (IHC) analyzes of TFIIA-alpha (E311) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
BiowMW	ca. 42 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42, Transcription initiation factor IIA alpha chain, TFIIA p35 subunit, Transcription initiation factor IIA beta chain, TFIIA p19 subunit, GTF2A1, TF2A1
Immunogen	Synthetic peptide, corresponding to amino acids 300-350 of Human TFIIA-alpha.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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