Item no. |
BS2224-50 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
50ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Cadherin2, Cadherin 2, Cadherin-2, CDw325, Neural cadherin, N-cadherin, CD325, CDH2, CDHN, NCAD |
Similar products |
N-Cadherin |
Available |
|
Swiss-Prot |
P19022 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200 |
Background |
Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2-terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxyterminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as beta-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P-cadherin and cadherin-5. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
N-cadherin (W745) pAb detects endogenous levels of N-cadherin protein. |
WB |
Western blot (WB) analysis of N-Cadherin (W745) pAb at 1:500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate |
IHC |
Immunohistochemistry (IHC) analyzes of N-cadherin (W745) pAb in paraffin-embedded human brain tissue. |
BiowMW |
ca. 100, 140 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Cadherin2, Cadherin 2, Cadherin-2, CDw325, Neural cadherin, N-cadherin, CD325, CDH2, CDHN, NCAD |
Immunogen |
Synthetic peptide, corresponding to amino acids 710-760 of Human N-Cadherin. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.