Item no. |
BS2053-50 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
50 ul |
Quantity options |
100 ul
50 ul
|
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IF, IHC |
Specific against |
Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus) |
Host |
Rabbit |
Conjugate/Tag |
Unconjugated |
Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase, Short name=hTDG, Thymine DNA glycosylase, hTDG |
Similar products |
TDG |
Available |
|
Application Notes |
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 |
Background |
In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch-specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitin-like proteins SUMO-1 and SUMO-2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity. |
Manufacturer - Product |
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
Manufacturer - Specificity |
TDG (K90) polyclonal antibody detects endogenous levels of TDG protein. |
Molecular Weight |
~ 46 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Immunogen |
Synthetic peptide, corresponding to amino acids 28-109 of Human TDG. |
Modification |
Unmodification |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.