Item no. |
BS1201-100 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
100ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Keratin, type I cytoskeletal 15, Cytokeratin-15, Short name=CK-15, Keratin-15, Short name=K15, KRT15, KRTB, Keratin 15, CK15, CK 15, Keratin15 |
Similar products |
Cytokeratin 15 |
Available |
|
Swiss-Prot |
P19012 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
Cytokeratin 15 (CK15, K15, K1CO, keratin15, type I cytoskeletal 15) is an intermediate filament (IF) type I protein that is responsible for the mechanical integrity of epithelial cells. Keratin family members are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains, and are clustered in a region on chromosome 17q21.2. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceus. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
Cytokeratin 15 (T12) pAb detects endogenous levels of Cytokeratin 15 protein. |
WB |
Western blot (WB) analysis of Cytokeratin 15 (T12) pAb at 1:500 dilution Lane1:HepG2 cell lysate Lane2:Mouse testis tissue lysate Lane3:Rat testis tissue lysate |
IHC |
Immunohistochemistry (IHC) analyzes of Cytokeratin 15 (T12) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 49 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Keratin, type I cytoskeletal 15, Cytokeratin-15, Short name=CK-15, Keratin-15, Short name=K15, KRT15, KRTB, Keratin 15, CK15, CK 15, Keratin15 |
Immunogen |
Synthetic peptide, corresponding to the N-terminus of Human Cytokeratin 15. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.