Item no. |
BS1152-50 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
50ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 5.1 |
32160702 |
ECLASS 6.1 |
32160702 |
ECLASS 8.0 |
32160702 |
ECLASS 9.0 |
32160702 |
ECLASS 10.0.1 |
32160702 |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Granzyme B, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, Short name=HLP, SECT, T-cell serine protease 1-3E, GZMB, CGL1, CSPB, CTLA1, GRB, Granzyme H, CCP- |
Similar products |
Granzyme B/H |
Available |
|
Swiss-Prot |
P10144/P20718 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
The Granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responses. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
Granzyme B/H (E20) pAb detects endogenous levels of Granzyme B and Granzyme H protein. |
WB |
Western blot (WB) analysis of Granzyme B/H (E20) pAb at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:Raw264.7 cell lysate Lane3:SP2/0 cell lysate Lane4:H9C2 cell lysate |
IHC |
Immunohistochemistry (IHC) analyzes of Granzyme B/H (E20) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 28 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Granzyme B, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, Short name=HLP, SECT, T-cell serine protease 1-3E, GZMB, CGL1, CSPB, CTLA1, GRB, Granzyme H, CCP-X, Cathepsin G-like 2, CTSGL2, Cytotoxic T-lymphocyte proteinase, Cytotoxic serine protease C, Short name=CSP-C, GZMH, CGL2, CTSGL2 |
Immunogen |
Synthetic peptide, corresponding to amino acids 1-50 of Human Granzyme B. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.