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C/EBP-beta (S229/182) polyclonal antibody

C/EBP-beta (S229/182) polyclonal antibody

Item no.	BS1037-50
Manufacturer	Bioworld Technology, Inc
Amount	50ul
Category	Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Neural Transcription Factors Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Polyclonal
Applications	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CCAAT/enhancer-binding protein beta, C/EBP beta, C/EBPbeta, C/EBP beta, Liver activator protein, LAP, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, Transcription factor 5, TCF-5, CEBPB, TCF5, ORF Names: PP9092
Similar products	C/EBP-beta
Available
Swiss-Prot	P17676
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200
Background	C/EBP beta is a member of the C/EBP transcription factor family. The C/EBP beta gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms four C/EBP beta isoforms. C/EBP beta is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP beta contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP beta gene that has direct implication for acute phase response in liver cells.The 35 kDa C/EBP beta form requires phosphorylation for its DNA binding ability, and increased binding of C/EBP beta isoforms during acute-phase reaction occurs through its upregulation and structural modification.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	C/EBP-beta (S229/182) pAb detects endogenous levels of total C/EBP-beta protein.
WB	Western blot (WB) analysis of C/EBP-beta (S229/182) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse brain tissue lysate Lane3:Rat kidney tissue lysate
IHC	Immunohistochemistry (IHC) analyzes of C/EBP-beta (S229/182) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
BiowMW	ca. 19, 36, 38 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	CCAAT/enhancer-binding protein beta, C/EBP beta, C/EBPbeta, C/EBP beta, Liver activator protein, LAP, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, Transcription factor 5, TCF-5, CEBPB, TCF5, ORF Names: PP9092
Immunogen	Synthetic peptide, corresponding to amino acids 200-250 of Human C/EBP-beta.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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