Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB |
Amount |
100ul |
Host |
Rabbit |
Item no. |
AP0410-100 |
Targets |
PRKCQ |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
Protein kinase C theta type, nPKC-theta, PRKCQ, PRKCT |
Swiss-Prot |
Q04759 |
Application all |
WB: 1:500ca.1:1000 |
Background |
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+. On the other hand, nPKC delta and epsilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
PKC theta (G532) pAb detects endogenous levels of PKC theta protein. |
WB |
Western blot (WB) analysis of PKC theta (G532) pAb at 1:500 dilution Lane1:Hela cell lysate treated with UV Lane2:NIH-3T3 cell lysate treated with UV Lane3:PC12 cell lysate treated with UV |
BiowMW |
ca. 82 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Protein kinase C theta type, nPKC-theta, PRKCQ, PRKCT |
Immunogen |
Synthetic peptide, corresponding to amino acids 520-570 of Human PKC theta. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.