Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse |
Applications |
WB, IHC |
Amount |
50ul |
Host |
Rabbit |
Item no. |
BS8233-50 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS |
Swiss-Prot |
Q9BVP2 |
Application all |
WB: 1:500ca.1:2000 IHC: 1:50ca.1:200 |
Background |
Nucleostemin, also designated Nucleolar GTP-binding protein 3, is a member of the MMR1/HSR1 GTP-binding protein family. It is expressed in the nucleoli of adult CNS stem cells, primitive bone marrow cells, embryonic stem cells and in several cancer cell lines. Nucleostemin is often used as a stem cell marker. Overexpression or depletion of the protein can reduce cell proliferation in CNS stem cells. Nucleostemin shuttles between the nucleus and the nucleolus and may be important in maintaining the proliferative capacity of stem cells. Nucleostemin is important in the growth regulation of liver cancer, gastric cancer and several other cancer types. The gene encoding Nucleostemin is localized to chromosome 3p21.1. |
Product |
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
GNL3 polyclonal antibody detects endogenous levels of GNL3 protein. |
WB |
WesternBlot (WB) analysis of GNL3 polyclonal antibody |
BiowMW |
ca. 62 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS |
Immunogen |
Recombinant full length Human GNL3. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.