Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse |
Applications |
WB, IHC |
Amount |
50ul |
Host |
Rabbit |
Item no. |
BS6982-50 |
Targets |
CD4 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3 |
Swiss-Prot |
P01730 |
Application all |
WB: 1:500ca.1:2000 IHC: 1:50ca.1:200 |
Background |
Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the beta2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation. |
Product |
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
CD4 pAb detects endogenous levels of CD4 protein. |
WB |
Western blot analysis of CD4 polyclonal antibody |
BiowMW |
ca. 51 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3 |
Immunogen |
Recombinant full length Human CD4. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.