Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse |
Applications |
WB, ICC |
Amount |
100ul |
Host |
Rabbit |
Item no. |
BS5899-100 |
Targets |
RHOG |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
Rho GDP-dissociation inhibitor 3, Rho GDI 3, Rho-GDI gamma, Rho GDIgamma, Rho-GDIgamma |
Swiss-Prot |
Q99819 |
Application all |
WB: 1:500ca.1:1000 ICC: 1:50ca.1:200 |
Background |
The Ras superfamily of small GTP-binding proteins are critical mediators of diverse cell signaling pathways, including those leading to proliferation, cytoskeletal organization and secretion. The counter-conversion of the active GTPbound form of these proteins to their inactive GDP-bound form is influenced by two types of regulatory proteins: those that alter the intrinsic GTPase activity of the GTP-binding proteins and those that alter the rate of GDP/GTP exchange. Guanine nucleotide-releasing factors (GRFs) increase the GDP dissociation rate, while GDP-dissociation inhibitors (GDIs) decrease the dissociation rate. The Rho GDI subfamily is composed of Rho GDIalpha, Ly-GDI (also known as Rho GDIbeta and previously known as GDI/D4) and Rho GDIgamma. The Rho GDI proteins interact with and have varying affinities for several Ras-like GTP binding proteins, including Rho A, Rho B, Rac and Cdc42. Ly-GDI is expressed only in hematopoietic cells, predominantly in B and T lymphocyte cell lines. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
ARHGDIG pAb detects endogenous levels of ARHGDIG protein. |
WB |
Western blot (WB) analysis of ARHGDIG pAb at 1:500 dilution Lane1:Jurkat cell lysate Lane2:Hela cell lysate Lane3:Raw264.7 cell lysate |
BiowMW |
ca. 25 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Rho GDP-dissociation inhibitor 3, Rho GDI 3, Rho-GDI gamma, Rho GDIgamma, Rho-GDIgamma |
Immunogen |
Synthetic peptide, corresponding to amino acids 176-221 of Human ARHGDIG. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.