Item no. |
AP0366-100 |
Manufacturer |
Bioworld Technology, Inc
|
Amount |
100ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB |
Specific against |
Human, Mouse, Rat, Fish |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Signal transducer and activator of transcription 3, Acute-phase response factor, STAT-3, APRF |
Similar products |
Stat3 |
Available |
|
Swiss-Prot |
P40763 |
Application all |
WB:1:500ca.1:1000 |
Background |
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-alpha and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3beta appears to be activated by both while Stat3alpha is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
Stat3 (S727) pAb detects endogenous levels of Stat3 protein. |
WB |
Western blot (WB) analysis of Stat3 (S727) pAb at 1:500 dilution Lane1:A549 whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate |
BiowMW |
ca. 88 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Signal transducer and activator of transcription 3, Acute-phase response factor, STAT-3, APRF |
Immunogen |
Synthetic peptide, corresponding to amino acids 700-750 of Human Stat3. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.