This kit provides calibration buffers and a protocol for producing a range of accurate calcium concentrations for the calibration of fluorescent Ca
2 indicators (1, 2). This product contains 50 mL each of components A and B:
Component A (zero free Ca
2 ): Zero mM CaEGTA (10 mM K
2EGTA, 100 mM KCI and 10 mM MOPS, pH 7.20)
Component B (40 uM free Ca
2 ): 10 mM CaEGTA (10 mM CaEGTA, 100 mM KCI and 10 mM MOPS, pH 7.20)
- Colorless liquids
- Store at 4°C
- Preservative-free, use within three months of receipt