Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K
d of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca
2 but with a significantly lower K
d than Fura-2 for Ca
2 . An important application of Mag-Fura-2 is its use in detecting high, transient Ca
2 concentration during Ca
2 spikes. Mag-Fura-2/tetrasodium salt can be loaded into cells by microinjection or scrape loading. Also see Mag-Fura-2, tetrapotassium salt and the cell membrane-permeant Mag-Fura-2 AM ester.
- Light yellow solid soluble in water (pH > 6)
- Store at 4°C and protect from light
- C18H10N2Na4O11
- MW: 523