Comparison

Biotin-16-dUTP, 1 mM In Ph 7.5 Tris-HCl

Item no. B-40022
Manufacturer Biotium
CASRN 136632-31-0
Amount 50 UL
Quantity options 50 UL 50 UG
Category
Type Nucleic acid label
Specific against other
ECLASS 10.1 32160414
ECLASS 11.0 32160414
UNSPSC 41105324
Shipping condition Cool pack
Available
Manufacturer - Category
Fluorescent Proteins, Nucleotides, Other Conjugates>Biotin, Streptavidin Conjugates|Fluorescent Proteins, Nucleotides, Other Conjugates>Labeled Nucleotides|Reactive CF® Dyes, Other Reactive Dyes, Biotinylation Reagents>Biotinylation Reagents
Shipping Temperature
Blue ice
Storage Conditions
-35°C to -5°C
Description
Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3' end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number '16' is the number of atoms in the linker between biotin and dUTP. Biotium also offers biotin-11-dUTP and biotin-20-dUTP. The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin. 
     
  • 1 mM solution in pH 7.5 Tris-HCl buffer
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  • Store at -20°C
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  • C32H48N7O18P3Sli4
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  • MW: 971.5
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  Biotin-16-dUTP is also available as a lyophilized solid.
Product origin
Synthetic
Undated stability guarantee in PI
12 months
Stability at RT during shipping (protected from light)
Stable for at least 1 month
UNSPSC Commodity Title
Conjugated nucleotides or oligomers

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 UL
Available: In stock
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