Bacterial Viability and Gram Stain Kit - 200 assays

Microbiology>Bacterial Viability|Microbiology>Fluorescent Gram Stains

The Bacterial Viability and Gram Stain Kit contains three components: CF® 488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria. CF® 488A-labeled wheat germ agglutinin stains the N-acetylglucosamine of the peptidoglycan layer of gram-positive bacteria with green fluorescence (Ex/Em 490/515 nm). The bacterial viability component of the assay is based on the integrity of the cell membrane. EthD-III is a nucleic acid binding dye that is membrane impermeable, which selectively stains cells with compromised plasma membranes with red fluorescence (Ex/Em 530/620 nm). All cells stain blue with the membrane permeable DNA dye DAPI (Ex/Em 358/461 nm). One kit is sufficient 200 assays when used according to the kit protocol.    The Bacterial Viability and Gram Stain Kit has been tested on the following bacterial species: Bacillus subtilis subsp. subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas fluorescens, and Staphylococcus epidermidis.

Lectin from wheat germ (Triticum aestivum)

12 months

Microbiology or bacteriology reagents or solutions or stains

12 months