Coelenterazine (ALSO KNOWN AS NATIVE COE

Enzyme Substrates>Luciferase Substrates

Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from ~0.1 uM to > 100 uM. Renilla luciferase has been widely used as a reporter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues. Also see water soluble Aquaphile™ Coelenterazine.

• Yellow solid soluble in MeOH or EtOH
• Store at -20° C and protect from light
• C₂₆H₂₁N₃O₃
• MW: 423
• [55779-48-1]

Store with desiccant; Protect from light

Stable; Color should be yellow, may contain dark red or brown flecks. If uniformly brown, it is oxidized and needs to be replaced.