Item no. |
BM-EKU03860 |
Manufacturer |
Biomatik
|
Amount |
96T |
Category |
|
Type |
Elisa-Kit |
Format |
96 well plate |
Applications |
ELISA |
Specific against |
Human |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Available |
|
Detection Range |
0.156-10 ng/mL |
Sensitivity |
0.056 ng/mL |
Sample Type |
Tissue homogenates, cell lysates and other biological fluids. |
Assay Time |
3 hours |
UniProt ID |
P13804 |
Gene ID |
2108 |
Gene Name |
Electron Transfer Flavoprotein Alpha Polypeptide |
Gene Aliases |
EMA; GA2; MADD; Glutaric Aciduria II |
Assay Type |
Sandwich |
Detection Method |
Colorimetric |
Shipping Condition |
Blue ice |
Storage Instructions |
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles. |
Precaution of Use |
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption. |
Expiry Date |
8 months |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). No significant cross-reactivity or interference between Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) and analogues was observed. |
Test Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Alpha Polypeptide (ETFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Assay Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Restrictions |
For research use only. Not for diagnostic procedures. |
Quality Systems |
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
Research Area |
Signal transduction; |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.