Human FLG-Ab (Anti-Filaggrin) ELISA Kit

FLG-Ab

1.56-100 ng/mL

Colorimetric

Sandwich

Short term: 4C; Long term: see manual.

6 months

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Human FLG-Ab . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for Human FLG-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the substrate solution is added to each well, only those wells that contain Human FLG-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FLG-Ab. The concentration of Human FLG-Ab in samples can be calculated by comparing the OD of the samples to the standard curve.

The kit is manufactured at ISO 9001 certified facilities.