Syphilis IgM ELISA Kit (96 Wells)

Principle of the assay: The OKDA00116 Syphilis IgM kit is based on the ELISA technique. In the assay, controls and unknowns are incubated in microtitration wells coated with purified and inactivated Treponema antigen. After incubation and washing, the wells are treated with the conjugate, composed of anti-human IgM antibodies labeled with peroxidase. After a second incubation and washing step, the wells are incubated with the substrate tetramethylbenzidine (TMB). An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by wavelength absorbance measurement at 450 nm. The absorbance measured is directly proportional to the concentration of anti-Treponema IgM antibodies present.